rabbit polyclonal anti-sdha  (Huabio Inc)

Journal: eLife

Article Title: Connexin 43 hemichannels regulate mitochondrial ATP generation, mobilization, and mitochondrial homeostasis against oxidative stress

doi: 10.7554/eLife.82206

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Article Snippet: Antibody , Anti-SDHA (rabbit polyclonal) , Invitrogen , Cat #459200, RRID: AB_1501830 , WB 1:1000, IF 1:1000.

Techniques: Knock-Out, Produced, Purification, Recombinant, Transfection, Sequencing, Software

Journal: iScience

Article Title: Prevention and regression of megamitochondria and steatosis by blocking mitochondrial fusion in the liver

doi: 10.1016/j.isci.2022.103996

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Article Snippet: Rabbit polyclonal anti-SDHA , Cell Signaling Technology , Cat# 11998; RRID: AB_2890191.

Techniques: Recombinant, Control, Western Blot, Protease Inhibitor, Membrane, Electron Microscopy, Activity Assay, Staining, Software

Journal: eLife

Article Title: Aging is associated with increased brain iron through cortex-derived hepcidin expression

doi: 10.7554/eLife.73456

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Article Snippet: Antibody , Rabbit polyclonal anti-SDHA antibody , Invitrogen , 45-920-0 , WB (1:1000).

Techniques: Protease Inhibitor, SYBR Green Assay, Immunoprecipitation, Bicinchoninic Acid Protein Assay, Isolation, Activity Assay, Sequencing, Software

Journal: Communications Biology

Article Title: Genome-wide CRISPR/Cas9 deletion screen defines mitochondrial gene essentiality and identifies routes for tumour cell viability in hypoxia

doi: 10.1038/s42003-021-02098-x

Figure Lengend Snippet: a Schematic diagram shows which datasets were compared in our analysis (hypoxia-glucose vs. normoxia-glucose). Sequencing reads from triplicate incubations were analysed by the MAGeCK analysis platform, and relative sgRNA abundances were calculated between experimental conditions. b Charts show FDR-corrected significance values of all sequenced genes, with significantly enriched (blue circles) or depleted (brown circles) sgRNAs in cells cultured in hypoxia-glucose compared to normoxia-glucose. n = 3. p < 0.05, FDR < 30%. c Pie charts show number of mitochondrial genes among the genes identified with significantly enriched or depleted sgRNAs from cells in ( a ). d Panel shows 31 selected mitochondrial genes with significantly enriched sgRNAs from ( b ). Genes significantly depleted in normoxia-glucose (plasmid vs. library) are highlighted in brown. e Western blots show expression of NDUFB10, SDHA, UQCRC2, COXIV, and BNIP3 in U2OS and HCT116 cells incubated for 5 days in normoxia (Nor) or hypoxia (Hyp, 1% O 2 ). β-Actin used as a loading control. f Chart shows overrepresentation analysis of all genes with significantly enriched sgRNAs in hypoxia (hypoxia-glucose vs. normoxia-glucose). Gene sets involving translation (mRNA splicing and processing of pre-mRNA), and regulation of actin cytoskeleton are highlighted (*). g , h Schematic diagram shows clusters of interacting genes with significantly enriched sgRNAs in hypoxia (hypoxia-glucose vs. normoxia-glucose) involved in mRNA processing ( g ), and cytoskeleton arrangement ( h ).

Article Snippet: Antibodies used were as follows, with dilutions in parentheses: rabbit polyclonal NDUFB10 (ab196019, 1:2000) from Abcam; rabbit polyclonal SDHA (11998, 1:1000) from Cell Signaling Technology; rabbit polyclonal UQCRC2 (ab14745, 1:1000) from Abcam; rabbit polyclonal COXIV (4850, 1:10,000) from Cell Signaling Technology; rabbit polyclonal BNIP3 (HPA003015, 1:2000) from Cambridge Biosciences; rabbit polyclonal SDHC (PA5-79966, 1:1000) from Invitrogen; mouse monoclonal β-Actin (ab6276, 1:10,000) antibody from Abcam; donkey anti-rabbit (NA934, 1:1000) and anti-mouse (NA931, 1:1000) horseradish peroxidase (HRP)-linked secondary antibodies from VWR.

Techniques: Sequencing, Cell Culture, Plasmid Preparation, Western Blot, Expressing, Incubation, Control